Sterilization, Washing, and Recycling of Contaminated Lab Plastics at Dunn School
Protocol modified from Alves et al., Access Microbiology 2021
In the User’s Laboratory:
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Carefully empty the contents of used tubes, vials, tissue culture flasks, and plates directly into Virkon solution without rinsing.
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Securely close the lids of all containers after disposal.
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For bacterial plates, remove the agar using a spatula and discard it into the autoclave box. Ensure that the spatula is decontaminated afterward by soaking it in 10% Chemgene for 10 minutes.
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Continue collecting the used tubes, vials, plates, and flasks in appropriate containers designated for contaminated plastic.
Washing up Facility:
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Collect the containers with contaminated plastics from the lab and transport them to the washing facility.
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Remove the lids from all tubes, vials, flasks, and plates.
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Prepare the 10% Chemgene solution by diluting the concentrate with tap water.
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Fully submerge the tubes, vials, flasks, plates, and their lids in a 10% Chemgene HLD4L Concentrate solution (Clear Unfragranced, 5 Litre XTM309-C STARLAB (UK) Ltd) for at least 10 minutes.
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The same solution of 10% Chemgene can be reused several times throughout the day but should be discarded at the end of each day.
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Thoroughly rinse all tubes, flasks, plates, vials, and lids with tap water after the disinfection process.
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Allow the tubes and lids to dry at room temperature overnight.
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Once dried, transfer the tubes and other items into designated recycling bags for proper recycling through Recycle-labs.
Contaminated lab plastic collection container
Signage for different containers at Dunn School
Bags filled with decontaminated and washed lab plastic ready for collection by Re-cycle lab
Contaminated lab plastic collection container